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Reporter options include NanoLuc®, firefly and Renilla luciferases, which have different protein sizes, reporter stabilities and brightness Detect the luciferase reporter using lytic or livecell reagents that provide varying levels of brightness and signal stability Dualreporter detection reagents offer normalization or assay multiplexing.
Renilla luciferase assay. Dieses als RenillaLuciferase bekannte Enzym ist kleiner (36 kDa) und emittiert blaues Licht Durch ihre Unterschiede in Substrataffinität und emittierter Wellenlänge können die klassische Luciferase und die RenillaLuciferase parallel in einem Test verwendet werden Bioluminiszente Bakterien besitzen ebenfalls eine Luciferase. Luciferase Reporter Gene Assays (9) Membrane Potential & Nerve Terminal Dyes Microbiology Microbiology Primary Antibodies (42) Bacterial Viability (5) Fluorescent Gram Stains (3) Other Bacterial Stains (10) Viability PCR Reagents (15) Firefly Assay Renilla Assay SteadyLuc. Die LuciferaseGene von Photinus pyralis und Renilla reniformis werden in der funktionellen Genforschung als Reportergene verwendet Dabei kommen beide Enzyme häufig im selben Ansatz zur Verwendung (dual luciferase assay) Wird das LuciferaseGen hinter einem Promotor in einen codierenden DNAAbschnitt eingefügt, so lässt die Stärke der.
The DualLuciferase® Reporter (DLR™) Assay System provides an efficient means of performing two reporter assays In the DLR™ Assay, the activities of firefly (Photinus pyralis) and Renilla (Renilla reniformis or sea pansy) luciferases are measured sequentially from a single sampleThe firefly luciferase reporter is measured first by adding Luciferase Assay Reagent II (LAR II) to generate. EvaGreen® Dye is a nextgeneration DNAbinding dye that is ideal for quantitative realtime PCR (qPCR) and many other applications Biotium scientists designed the dye by taking into consideration several essential dye properties relevant to PCR, including PCR inhibition, safety, stability and fluorescence spectra of the dye. Invitrogen Alexa Fluor 594 dye is a bright, redfluorescent dye that can be excited using the 561 nm or 594 nm laser lines For stable signal generation in imaging and flow cytometry, Alexa Fluor 594 dye is pHinsensitive over a wide molar range.
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